Silver binding nucleolar organizing regions in Oral Leukoplakia and Oral Squamous Cell Carcinoma – a clinical histopathological study
نویسنده
چکیده
Background: Nucleolar organizing regions (NORs) are associated with proliferative activity and represents as a diagnostic aid in several neoplastic entities. Objective: The purpose of this study was to determine and compare the AgNOR (Silver binding nucleolar organizing region) count in healthy oral mucosa, oral leukoplakia and oral squamous cell carcinoma (OSCC). Methods: The biopsied tissue specimens were obtained from 15 cases of oral leukoplakia, 15 cases of oral squamous cell carcinoma and 20 cases of normal healthy epithelium followed by staining with silver-nitrate method for the nucleolar organizing regions. Further AgNOR dots were counted by the criteria under 100X oil immersion objective. Results: The mean AgNOR count showed a gradual increase from normal epithelium to leukoplakia to OSCC which was found to be statistically significant. Conclusion: The AgNOR method can be used to provide information on the malignant potential in premalignant lesions and aggressiveness of the malignant lesions.
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Silver Binding Nucleolar Organizer Regions Dots in Oral Leukoplakia with Epithelial Dysplasia and Oral Squamous Cell Carcinoma: An In Vivo Study
Silver binding nucleolar organizer regions (AgNOR) in normal oral mucosa (NOM), oral leukoplakia with epithelial dysplasia (ED), and oral squamous cell carcinoma (OSCC) were studied. The mean AgNOR count per nucleus increased from NOM to ED to OSCC. Tissue showing ED in oral leukoplakia and OSCC cases showed higher counts, wider scatter, and smaller size of AgNOR dots in the nuclei. The study s...
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